Extract rau ntawm Huanglian, ib lam tshuaj ntsuab, Induces Cell hlob ntes thiab Apoptosis los Upregulation Interferon-β thiab TNF-α nyob hauv tib neeg lub mis mob hlwb

Huanglian (Coptidis rhizoma), tshuaj ntsuab hauv tsoos suav tshuaj, siv lug tau pom tias nyuam qhuav muaj anticancer kev ua ub no. Txawm li ntawd los, cov molecular mechanism underlying cov nyhuv anticancer ntawm cov tshuaj ntsuab no tsis to taub. No, seb huanglian extract muaj feem xyuam rau cov kev qhia txog kev mob nkeeg noob twb tsis tau hais tseg. Txoj kev tshawb no siv DNA microarray tshuab los saib tus ntxim ntawm lub herbal extract rau cov qhia lub noob uas txuam nrog carcinogenesis rau ob tug neeg mis kheesxaws ntawm kab, cov ER-zoo MCF-7 thiab ER-tsis MDA-MB-231 hlwb. Kev kho mob uas lub hlwb mob cancer nrog huanglian extract markedly inhibited lawv proliferation yam koob - thiab lub sij hawm-his. Txoj kev loj hlob inhibitory nyhuv heev dua no: nyob rau MCF-7 ntawm kab dua uas nyob rau hauv lub hlwb MDA-MB-231. DNA microarray pom ices tias tus huanglian dramatically si mRNA qhia interferon-β (IFN-β) thiab lub pob necrosis tau-α nyob hauv hlwb MCF-7. Tsom xam quantitative lam real-time los yog blotting thaj paub tseeb hais tias cov upregulation ntawm hauv ob tug noob (tshwj xeeb yog IFN-β) rau hauv hlwb MCF-7, tiam sis tsis nyob hauv hlwb MDA-MB-231. Sib ntxiv ntawm neutralizing antibody tiv thaiv IFN-β rau nruab nrab culture markedly inhibited tus antiproliferative huanglian hob nyhuv, lees tias cov koom tes ntawm IFN-β nyob rau hauv cov nyhuv rau huanglian thiab kuj suggesting ib autocrine chaw rau qhov txiav txim ntawm IFN-β nyob rau hauv cov chaw no. Given tias IFN-β yog cov tseem ceeb tshaj anticancer cytokines, tus upregulation ntawm no noob los huanglian yog, tsawg kawg yog nyob sab, lub luag hauj lwm rau nws cov antiproliferative nyhuv. Cov qhabnias ntawm txoj kev tshawb no implicate huanglian li pheej muaj tshuaj ntsuab rau chemoprevention thiab cov kws khomob ntawm tej tawv nqaij.